Concentration Assay:Article Title:
Article Snippet: .. Table 20: Stre tococcus a alactiae Isolates Tested Organism _ Isolate ID (Strain) Test Concentration Result _ (copies/mL) xLoD Streptococcus agalactiae ATCC 13813 (G19) 1.9E+04 lx Streptococcus agalactiae Detected ATCC 12403 [D136C(3)] 5.7E+04 3x ATCC BAA-2669 (5030-08) 5.7E+04 3x CI 2460 5.7E+04 3x ATCC BAA-611 (2603 V/R) 5.7E+04 3x ATCC 12386 (090R) 5.7E+04 3x , Organism Isolate ID (Strain) Test Concentration Result (copies/mL) xLoD Streptococcus pyogenes ATCC 19615 (Bruno) 8.9E+03 lx Streptococcus pyogenes Detected ATCC 49399 (QC A62) 2.7E+04 3x ATCC 12344 (T1) 2.7E+04 3x ATCC 12348 (S43) 2.7E+04 3x ATCC 700294 (SF370;M1 GAS) 2.7E+04 3x ATCC BAA-947 (MGAS 5005) 2.7E+04 3x ATCC 12384 (C203) 2.7E+04 3x P-03-0543 804 IS0a 8.9E+05 100x Streptococcus pyogenes No t Detected aIsolate was from a clinical specimen; a deletion in the gene target was identified that prevents amplification/detection Table 23: Bacteroides fragilis Isolates Tested Isolate ID (Strain) Organism DEN200066 - Page 27 of 84 Bacteroides fragilis ATCC 25285 (VPI 2553) 1.1E+03 lx Bacteroides fragilis Detected ATCC BAA-2283 (2-1-56 FAA) 3.3E+03 3x ATCC 29768 (12256/P8) 3.3E+03 3x ATCC 29771 (2044 [CDC 1261; M-488]) 3.3E+03 3x ATCC 43937 (F1355 [WAL 78-189A]) 3.3E+03 3x Citrobacter cronae no se. uence/not tested a/n si/ico analysis identified sequence variation that is predicted to impact reactivity in 4/6 (50%) C. werkmanii sequences Not Detected Enterobacter cloacae" AR Bank #0154 1.3E+05 lx Enterobacter cloacae Detected NCTC 13464 3.9E+05 3x Enterobacter cloacae ssp. cloacae" ATCC 13047 (CDC 442-68) 3.9E+05 3x ATCC 222 (CDC 435) 3.9E+05 3x Enterobacter cloacae ssp. dissolvensa ATCC 23373D-5 gDNA (ICPB ED105) 3.9E+05 3x Enterobacter hormaechei ATCC BAA-2082 3.9E+05 3x ATCC 700323 3.9E+05 3x Enterobacter hormaechei ssp. hormaechei ATCC 49162 1.3E+07 100x Enterobacter hormaechei ssp. oharea CCUG 53905T 3.9E+05 3x Enterobacter hormaechei ssp. steigerwaltii CCUG 53904T 3.9E+05 3x Enterobacter hormaechei ssp. xiangfangensis DSM 46348 3.9E+05 3x Enterobacter kobei GRE 1753004 3.9E+05 3x Enterobacter ludwigii" DSM 16688 (EN-119) 3.9E+05 3x CCUG 23050 3.9E+05 3x Enterobacter mori DSM 26271 (R18-2) 3.9E+05 3x Enterobacter roggenkampii DSM 16690 (EN-117) 3.9E+05 3x aEnterobacter asburiae isolate ATCC 35953 has sequence variation under assay primers that impairs detection at 100x LoD and lower. .. A similar impact on reactivity is predicted for 6/76 (7.9%) Enterobacter asburiae sequences evaluated and impaired amplification and detection is also predicted for a subset of E. cloacae (8/516, 1.6%) and E. ludwigii (2/25, 8.0%) sequences. bE. hormaechei ssp. hormaechei isolate ATCC 49162 has sequence variation under assay primers that impairs detection at 10x LoD and lower.
Amplification:Article Title:
Article Snippet: .. Table 20: Stre tococcus a alactiae Isolates Tested Organism _ Isolate ID (Strain) Test Concentration Result _ (copies/mL) xLoD Streptococcus agalactiae ATCC 13813 (G19) 1.9E+04 lx Streptococcus agalactiae Detected ATCC 12403 [D136C(3)] 5.7E+04 3x ATCC BAA-2669 (5030-08) 5.7E+04 3x CI 2460 5.7E+04 3x ATCC BAA-611 (2603 V/R) 5.7E+04 3x ATCC 12386 (090R) 5.7E+04 3x , Organism Isolate ID (Strain) Test Concentration Result (copies/mL) xLoD Streptococcus pyogenes ATCC 19615 (Bruno) 8.9E+03 lx Streptococcus pyogenes Detected ATCC 49399 (QC A62) 2.7E+04 3x ATCC 12344 (T1) 2.7E+04 3x ATCC 12348 (S43) 2.7E+04 3x ATCC 700294 (SF370;M1 GAS) 2.7E+04 3x ATCC BAA-947 (MGAS 5005) 2.7E+04 3x ATCC 12384 (C203) 2.7E+04 3x P-03-0543 804 IS0a 8.9E+05 100x Streptococcus pyogenes No t Detected aIsolate was from a clinical specimen; a deletion in the gene target was identified that prevents amplification/detection Table 23: Bacteroides fragilis Isolates Tested Isolate ID (Strain) Organism DEN200066 - Page 27 of 84 Bacteroides fragilis ATCC 25285 (VPI 2553) 1.1E+03 lx Bacteroides fragilis Detected ATCC BAA-2283 (2-1-56 FAA) 3.3E+03 3x ATCC 29768 (12256/P8) 3.3E+03 3x ATCC 29771 (2044 [CDC 1261; M-488]) 3.3E+03 3x ATCC 43937 (F1355 [WAL 78-189A]) 3.3E+03 3x Citrobacter cronae no se. uence/not tested a/n si/ico analysis identified sequence variation that is predicted to impact reactivity in 4/6 (50%) C. werkmanii sequences Not Detected Enterobacter cloacae" AR Bank #0154 1.3E+05 lx Enterobacter cloacae Detected NCTC 13464 3.9E+05 3x Enterobacter cloacae ssp. cloacae" ATCC 13047 (CDC 442-68) 3.9E+05 3x ATCC 222 (CDC 435) 3.9E+05 3x Enterobacter cloacae ssp. dissolvensa ATCC 23373D-5 gDNA (ICPB ED105) 3.9E+05 3x Enterobacter hormaechei ATCC BAA-2082 3.9E+05 3x ATCC 700323 3.9E+05 3x Enterobacter hormaechei ssp. hormaechei ATCC 49162 1.3E+07 100x Enterobacter hormaechei ssp. oharea CCUG 53905T 3.9E+05 3x Enterobacter hormaechei ssp. steigerwaltii CCUG 53904T 3.9E+05 3x Enterobacter hormaechei ssp. xiangfangensis DSM 46348 3.9E+05 3x Enterobacter kobei GRE 1753004 3.9E+05 3x Enterobacter ludwigii" DSM 16688 (EN-119) 3.9E+05 3x CCUG 23050 3.9E+05 3x Enterobacter mori DSM 26271 (R18-2) 3.9E+05 3x Enterobacter roggenkampii DSM 16690 (EN-117) 3.9E+05 3x aEnterobacter asburiae isolate ATCC 35953 has sequence variation under assay primers that impairs detection at 100x LoD and lower. .. A similar impact on reactivity is predicted for 6/76 (7.9%) Enterobacter asburiae sequences evaluated and impaired amplification and detection is also predicted for a subset of E. cloacae (8/516, 1.6%) and E. ludwigii (2/25, 8.0%) sequences. bE. hormaechei ssp. hormaechei isolate ATCC 49162 has sequence variation under assay primers that impairs detection at 10x LoD and lower.
Sequencing:Article Title:
Article Snippet: .. Table 20: Stre tococcus a alactiae Isolates Tested Organism _ Isolate ID (Strain) Test Concentration Result _ (copies/mL) xLoD Streptococcus agalactiae ATCC 13813 (G19) 1.9E+04 lx Streptococcus agalactiae Detected ATCC 12403 [D136C(3)] 5.7E+04 3x ATCC BAA-2669 (5030-08) 5.7E+04 3x CI 2460 5.7E+04 3x ATCC BAA-611 (2603 V/R) 5.7E+04 3x ATCC 12386 (090R) 5.7E+04 3x , Organism Isolate ID (Strain) Test Concentration Result (copies/mL) xLoD Streptococcus pyogenes ATCC 19615 (Bruno) 8.9E+03 lx Streptococcus pyogenes Detected ATCC 49399 (QC A62) 2.7E+04 3x ATCC 12344 (T1) 2.7E+04 3x ATCC 12348 (S43) 2.7E+04 3x ATCC 700294 (SF370;M1 GAS) 2.7E+04 3x ATCC BAA-947 (MGAS 5005) 2.7E+04 3x ATCC 12384 (C203) 2.7E+04 3x P-03-0543 804 IS0a 8.9E+05 100x Streptococcus pyogenes No t Detected aIsolate was from a clinical specimen; a deletion in the gene target was identified that prevents amplification/detection Table 23: Bacteroides fragilis Isolates Tested Isolate ID (Strain) Organism DEN200066 - Page 27 of 84 Bacteroides fragilis ATCC 25285 (VPI 2553) 1.1E+03 lx Bacteroides fragilis Detected ATCC BAA-2283 (2-1-56 FAA) 3.3E+03 3x ATCC 29768 (12256/P8) 3.3E+03 3x ATCC 29771 (2044 [CDC 1261; M-488]) 3.3E+03 3x ATCC 43937 (F1355 [WAL 78-189A]) 3.3E+03 3x Citrobacter cronae no se. uence/not tested a/n si/ico analysis identified sequence variation that is predicted to impact reactivity in 4/6 (50%) C. werkmanii sequences Not Detected Enterobacter cloacae" AR Bank #0154 1.3E+05 lx Enterobacter cloacae Detected NCTC 13464 3.9E+05 3x Enterobacter cloacae ssp. cloacae" ATCC 13047 (CDC 442-68) 3.9E+05 3x ATCC 222 (CDC 435) 3.9E+05 3x Enterobacter cloacae ssp. dissolvensa ATCC 23373D-5 gDNA (ICPB ED105) 3.9E+05 3x Enterobacter hormaechei ATCC BAA-2082 3.9E+05 3x ATCC 700323 3.9E+05 3x Enterobacter hormaechei ssp. hormaechei ATCC 49162 1.3E+07 100x Enterobacter hormaechei ssp. oharea CCUG 53905T 3.9E+05 3x Enterobacter hormaechei ssp. steigerwaltii CCUG 53904T 3.9E+05 3x Enterobacter hormaechei ssp. xiangfangensis DSM 46348 3.9E+05 3x Enterobacter kobei GRE 1753004 3.9E+05 3x Enterobacter ludwigii" DSM 16688 (EN-119) 3.9E+05 3x CCUG 23050 3.9E+05 3x Enterobacter mori DSM 26271 (R18-2) 3.9E+05 3x Enterobacter roggenkampii DSM 16690 (EN-117) 3.9E+05 3x aEnterobacter asburiae isolate ATCC 35953 has sequence variation under assay primers that impairs detection at 100x LoD and lower. .. A similar impact on reactivity is predicted for 6/76 (7.9%) Enterobacter asburiae sequences evaluated and impaired amplification and detection is also predicted for a subset of E. cloacae (8/516, 1.6%) and E. ludwigii (2/25, 8.0%) sequences. bE. hormaechei ssp. hormaechei isolate ATCC 49162 has sequence variation under assay primers that impairs detection at 10x LoD and lower.
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